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METHOD OF MOLECULAR-GENETIC IDENTIFICATION OF WOODY PLANT SPECIES POPULATIONS
METHOD OF MOLECULAR-GENETIC IDENTIFICATION OF WOODY PLANT SPECIES POPULATIONS
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机译:木本植物种群分子遗传鉴定的方法
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摘要
FIELD: agriculture.;SUBSTANCE: invention relates to the field of biochemistry, in particular to a method of molecular-genetic identification of woody plant species, which includes the choice of efficient stable molecular markers, collecting material, molecular genetic analysis using PCR, analysis of revealed ISSR-markers and detection of identification (monomorphic and polymorphic) ones, analysis of the obtained data after sequencing, creation of molecular-genetic formula, creation of barcode, creation of genetic passport. The method is characterised in that in the molecular-genetic formula and the barcode in addition to identification DNA fragments of different sizes, amplified as a result of the polymerase chain reaction (PCR), other structural changes of genomes are made, such as deletions, duplications, single nucleotide substitutions (SNP-Single nucleotide Polymorphism), identified in the comparative analysis of nucleotide sequences after sequencing of genomic DNA.;EFFECT: invention enables to identify effectively the woody plant species at the nucleotide level.;7 cl, 1 dwg
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