METHODS FOR OBTAINING HAIR MICROFOLLICLES AND DE NOVO PAPILLAE AND THEIR APPLICATION FOR IN VITRO TESTS AND IN VIVO IMPLANTATIONS

摘要

FIELD: biotechnologies.;SUBSTANCE: method for obtaining hair microfollicle of mammal includes the following stages: de novo papilla and another cell population chosen from the group including fibroblasts, keratinocytes and/or melanocytes are provided, and de novo papilla is incubated together with the other cell population under conditions of unattached culture. Obtaining de novo papillae includes the following: dermal papilla from mammal hair follicle is provided; fibroblasts of dermal hair papilla are extracted from dermal papilla by mechanical fixing of the dermal papilla on the surface of vessel for cell culture, at that basal plate is punched to provide extraction of dermal hair papillae; extracted dermal hair papillae are thickened in monolayer culture without collagen-coated surface, where the dermal hair papillae are subcultivated; thickened dermal hair papillae are concentrated in cell families, which indicate size and form of physiologic dermal papillae, where the dermal hair papillae are differentiated in vessels without culture adhesion under concentration of cells on the vessel surface within range of 1000-100000 of dermal hair papillae per cm², and de novo papillae are covered with matrix proteins.;EFFECT: inventions allow formation of hair microfollicle capable of beard hair formation.;16 cl, 4 dwg, 5 tbl, 6 ex