METHOD FOR OBTAINING IMMUNOGENIC COMPOSITION BASED ON Ag85A-DBD HYBRID PROTEIN AND DEXTRANE; pAg85A-DBD RECOMBINANT PLASMIDE; Escherichia coli pREP4, pAg85A-DBD STRAIN; Ag85A-DBD CHIMERIC PROTEIN

摘要

FIELD: biotechnologies.;SUBSTANCE: invention represents Escherichia coli M15 [pREP4, pAg85A-DBD] strain - producer of Ag85A-DBD chimeric protein, as well as a method for immobilisation, concentration and cleaning of protein obtained based on dextrane. Invention relates to a method for obtaining immunogenic composition based on Ag85A-DBD recombinant protein mixed with dextrane and to Ag85A-DBD recombinant protein itself.;EFFECT: invention allows obtaining strain - producer providing high level of production of stable immunogenic proteins that can be obtained, immobilised and cleaned at a single stage, and obtaining effective immunogenic compositions against tuberculosis.;4 cl, 2 dwg, 1 tbl, 5 ex