METHOD FOR TYPING TISSUE-SPECIFIC TRANSCRIPTION FACTOR HNF4α ISOFORMS IN HUMAN HEPATOCELLULAR CARCINOMAS

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine and concerns a method for typing tissue-specific transcription factor HNF4α isoforms in human hepatocellular carcinomas. The method represents an immune histochemical study with using monoclonal antibodies to HNF4α isoform types and is characterised by the fact that for detecting antigens a two-stage high-sensitive polymeric peroxidase system is used. A retrieval procedure is performed in a citrate buffer at pH 6.0 and t=95°; an incubation with primary antibodies uses a high dilution of HNF4αP2 and HNF4αP1 antibodies - 1.5 mcg/ml and 3 mcg/ml respectively.;EFFECT: invention provides high specificity and sensitivity of the method, high-accuracy assessment of colour density of the tumour cell nuclei, a degree of the tumour cells differentiation and their malignancy as shown by an expression level of HNF4α isoform types, reduced time of the study up to 4 hours.;3 ex, 4 dwg