METHOD FOR DETERMINING FUNCTIONAL ACTIVITY OF IONISED CALCIUM IN HUMAN BLOOD SERUM

摘要

FIELD: medicine.;SUBSTANCE: invention can be used for determining functional activity of ionised calcium (FAI Ca²⁺) in human blood serum. The method for determining FAI Ca²⁺ is based on conducting a bovine erythrocyte lysis reaction in 10% human blood serum at temperature 37°C for 10 min in the presence of 0.55 mM ethylene glycol tetraacetic acid in a buffer for 10 min. An incubation is followed by determining a degree of inhibition of erythrocyte lysis; if observing a complement activity inhibition of less than 30%, a high functional activity of ionised calcium in a human body is stated; the inhibition of 31% to 70% shows the normal activity, and the inhibition of more than 71% - the low activity.;EFFECT: using easy and fast to implement method enables detecting preclinical conditions of immune deficiency, can be used as a specific marker for an immunomodulatory therapy, provides conducting the in-depth study of autoimmune pathogenesis, ensuring early prevention thereof and controlling the effectiveness of the conducted therapy.;5 ex, 6 tbl