FUSED PROTEIN OF THIOREDOXIN AND INFESTIN 4 DOMAIN, METHOD FOR PREPARING IT, EXPRESSION PLASMID DNA CODING FUSED PROTEIN, AND BACTERIUM Escherichia coli TRANSFORMED BY THIS PLASMID DNA

摘要

FIELD: medicine.;SUBSTANCE: inventions refer to biotechnology and concern a fused protein for the specific inhibition of blood coagulation, an expression plasmid DNA coding this fused protein, a bacterium of the genus Escherichia transformed by this DNA, and to a method for preparing the fused protein. The presented fused protein contains thioredoxin I E.coli and infestine-4 and is characterised by the sequence SEQ ID NO:2. The plasmid DNA contains the sequence SEQ ID NO:1 coding the presented fused protein and controlled by a promoter functioning in a bacterial cell. The method for preparing the above fused protein involves culturing the above bacteria in a nutrient medium, breaking the bacterial cells and purifying the above fused protein with using a metal chelate chromatography and an anion-exchange chromatography.;EFFECT: characterised solutions enables preparing the protein providing the above specificity to be used in blocking the contact activation of blood coagulation by inhibition of the XIIa factor and the absence of inhibition of the Xa factor.;4 cl, 10 dwg, 7 ex