METHOD OF DETERMINING SIMULTANEOUSLY PRESENT FAT-SOLUBLE VITAMINS A, D2, E AND ?-CAROTENE BY THIN-LAYER CHROMATOGRAPHY

摘要

FIELD: chemistry.;SUBSTANCE: method of determining fat-soluble vitamins A, D₂, E and β-carotene which are present at the same time includes separating the fat-soluble vitamins from a substance by extraction with 96% ethanol, separating the alcohol extract of vitamins using a separating funnel, successive chromatography using Sorbfil PTSKH-P-A silica gel plates on a polymer substrate using two eluents with a different range, time of saturating the chamber with eluent vapour of 20 minutes and elution time of 55 min; drying the plates at temperature not lower than 80°C in a temperature-controlled chamber for 3-5 min, treating the plates with a developer - 5% alcohol solution of phosphatomolybdic acid; according to the invention, the eluents used are hexane:chloroform (19:1) and hexane:chloroform (3:1), and detection of the chromatographic zone of β-carotene is carried out before treating the plates with a developer in day light.;EFFECT: simpler and faster process of determining fat-soluble vitamins.;10 dwg, 3 ex