METHOD TO CULTIVATE SUBLIMATED STRAINS OF MICROORGANISMS

摘要

FIELD: biotechnologies.;SUBSTANCE: method to cultivate sublimated strains of microorganisms includes introduction of a growth stimulant and a source of carbon into a dense nutrient medium with subsequent seeding of microorganism cells, incubation of seeds and accounting of viable microbial cells. Sublimated strains of microorganisms include Yersinia pestis EV, Escherichia coli C600, Bacillus anthracis "СТИ", Vibrio cholerae NAG 504, Brucella abortus 19BA. The growth stimulant and carbon source is 96% ethyl alcohol in the amount of 1.0-2.0% of the medium volume. If necessary, they add gentian violet in concentration of 1:100000.;EFFECT: improved yield of viable strain cells on dense nutrient media.;1 tbl, 5 ex