FUNGAL STRAIN Penicillium verruculosum B10 EGII PRODUCER OF ENDO-1,3/1,4-β-GLUCANASE, CELLULASE, β-GLUCOSIDASE AND XYLANASE AND METHOD OF PRODUCTION OF FODDER COMPLEX ENZYME PREPARATION

摘要

FIELD: biotechnology.;SUBSTANCE: inventions relate to a strain of the fungus Penicillium verruculosum B10 EGII and a method of production of the fodder complex enzyme preparation. The presented strain is a producer of endo-1.3/1.4-β-glucanase, cellulase, β-glucosidase and xylanase. It is obtained on the basis of a strain Penicillium verruculosum RNCM F-3764D, by transforming with its plasmids pSTA10 and pPrCBHI-EGII. In the plasmid pPrCBHI-EGII the nucleotide sequence of the structural gene of endo-1.3/1.4-β-glucanase of Penicillium verruculosum is aligned with nucleotide sequences of the promoter region, the signal peptide and the terminator of gene cbhI of cellobiohydrolase I Penicillium verruculosum. The method of production of the preparation containing endo-1.3/1.4-β-glucanase, cellulase, β-glucosidase and xylanase comprises cultivation of the strain of fungus Penicillium verruculosum B10 EGII on the optimised nutrient medium in the fermenter and filtration, ultrafiltration and lyophilisation of the culture liquid.;EFFECT: inventions can be used to produce fodder complex enzyme preparation enriched with endoglucanase.;2 cl, 1 dwg, 2 tbl, 5 ex