A method for the recognition of sepsis and / or sepsis-like states

摘要

A process for the in vitro detection of sepsis,characterized in thatit comprises the following steps:a) isolating a plurality of sample-rnas originating from the group consisting of a human blood sample;a1) adding a cdna, which for sepsis specific gene or gene fragment isb) marking the sample-rna and / or of the cdna with a detectable marker;c) contacting the sample-rna with the cdna under hybridization conditions;d) contacting of control-rna, which a control without sirs / sepsis, with at least of a cdna under hybridization conditions;e) quantitative detection of the marker signals of the hybridized sample-rna and the control-rna;f) comparing the quantitative data of the marker signals in order to make a statement as to whether for sepsis specific genes or gene fragments in the sample are expressed to a greater or lesser extent than in the control;g) where sepsis is present when the totality of the following group of genes or gene fragments with the seq id no: 325, 268, 294, 235, 262, 240, 248, 331, 486, 251, 903, 196, 324, 672, 134, 363, 259, 168, 326, 671, 114, 253, 116, 489,485, 273, 154, 187, 358, 495, 330, 327, 1511, 314, 339, 219, 328, 348, 272, 232, 349, 310, 65, 265, 361, 269, 652, 1755, 151, 275, 66, 317, 329 is overexpressed;and / orthe totality of the following group of genes or gene fragments with the seq id no: 2050, 375, 211, 250, 1477, 130, 23, 46, 26, 764, 383, 298, 28, 146, 1352, 42, 257, 1502, 203, 19, 12, 95, 188, 47, 45, 237, 706, 129, 1353, 137, 51, 43, 169, 1462, 59, 378, 246, 201, 67, 1597, 216, 490, 5, 93, 142, 189, 1442, 152, 53, 238, 180, 491, 190, 148, 386, 245 underexpresses is.