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Confocal laser-scanning microscope for examining a sample
Confocal laser-scanning microscope for examining a sample
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机译:共聚焦激光扫描显微镜检查样品
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摘要
Confocal laser-scanning microscope (20) for examining a sample (46), with a light source (22), which generates a illumination light beam (24),a scanning unit (38), the illuminating light beam (24) in such a way that it deflects the sample (46) is optically scans,a main beam splitter (34), the illuminating light beam (24) from the specimen (46) extending detection light (48) separates,a detection pinhole (50), as a result of the at least partially from the illuminating light beam (24) of the separate detection light (48),at least two detector units (102, 104, 106), which the by the detection pinhole (50) detection light (48) is adapted to detect,and, with an optical element (62), which in the beam direction between the detection pinhole (50) and the detector units (102, 104, 106) is arranged, and in which the detection light (48) in at least two beam (70, 72, 74) and within the bundle of rays (70, 72, 74) is split spectrally.
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