Detection method for Mycobacterium avium ssp. paratuberculosis

摘要

The present application relates to a method for the specific detection and optionally for the quantification of Mycobacterium avium ssp. paratuberculosis (MAP) in a sample of an individual. According to the method of the invention, the detection of the presence of the IS900 region in a sample is carried out with the aid of a nucleic acid amplification and specific oligonucleotides. In a further aspect, a test kit for the specific detection of MAP in a sample by means of amplification methods is provided herein. Finally, specific oligonucleotides suitable for the specific detection of MAP are disclosed.