Marker substance for use in detection reagent of immunoassay for specific detection of biomaterial, has hetero element, subunit that is peptide, and reporter group that comprises chelating agent, complexing agent and ion exchanger

摘要

The marker substance has a reactive group and a carrier material having two pieces of a subunit. The pieces are connected with one another by a peptide bond. The sub-unit has amino acid with a hetero element-containing reporter groups. The hetero element is any chemical element other than hydrogen, carbon, nitrogen and oxygen. The subunit is a peptide. The reporter group comprises a chelating agent, a complexing agent and ion exchanger. A substrate is provided a thiocarbonyl group or a thioether bond. Independent claims are included for the following: (1) a method for preparing a marker substance; (2) a detection reagent comprises molecule or fragment of a molecule selected under an antibody that comprises immunoglobulin G, immunoglobulin M, immunoglobulin E, avidin, biotin, enzyme, enzyme substrate, hormone, protein A, protein G, receptor and streptavidin; (3) an analysis method for qualitative and quantitative detection of an analyte in a sample; (4) an immunoassay selected from enzyme immunoassay, enzyme-linked immunosorbent assay, radioimmunoassay, immunoblot, western blot, southern blot or microarray; and (5) a method for retracking and covered individual marking of a biomolecule.