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DETECTION LIGHT ENHANCING METHOD USING LIGHT REFLECTIVE MATERIAL IN IMMUNO-CHROMATOGRAPHY

机译:免疫层析法中使用光反射材料的检测光增强方法

摘要

PROBLEM TO BE SOLVED: To provide an immuno-chromatographic specimen which achieves both high sensitivity detection of a substance to be detected and a simple specimen structure, although these items are not compatible normally.SOLUTION: Provided is an immuno-chromatographic specimen which: contains a membrane on which capture substances, being ligands bonding with a substance to be detected, are immobilized; uses insoluble carrier particles which couple the ligands bonding with the substance to be detected; accumulates the insoluble carrier particles by making the capture substances immobilized on the membrane capture the insoluble carrier particles; radiates light on the membrane; and detects light emitted from a site where the insoluble carrier particles are accumulated or from a surrounding area excluding the site where the insoluble carrier particles are accumulated, in order to measure the substance to be detected. The immuno-chromatographic specimen is provided with a light reflective material on the opposite side of a membrane side irradiated with light.
机译:解决的问题:提供一种免疫色谱标本,虽然通常不兼容,但可以实现对待检测物质的高灵敏度检测和简单的标本结构。解决方案:提供的免疫色谱标本包括:固定有与被检测物质结合的配体的捕获物质的膜。使用不溶性载体颗粒,该颗粒将配体与待检测物质结合在一起;通过使固定在膜上的捕获物质捕获不溶性载体颗粒来积累不溶性载体颗粒;在膜上辐射光;并检测从不溶性载体颗粒积聚的部位或除了不溶性载体颗粒积聚的部位以外的周围区域发射的光,以测量要检测的物质。免疫色谱样品在被光照射的膜侧的相反侧上具有光反射材料。

著录项

  • 公开/公告号JP2015031610A

    专利类型

  • 公开/公告日2015-02-16

    原文格式PDF

  • 申请/专利权人 DENKA SEIKEN CO LTD;

    申请/专利号JP20130161691

  • 申请日2013-08-02

  • 分类号G01N33/543;G01N21/64;G01N21/78;

  • 国家 JP

  • 入库时间 2022-08-21 15:33:47

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