Method for incorporating amino acid containing BCN group into polypeptide using orthogonal codon encoding amino acid containing BCN group and orthogonal PylRS synthase

摘要

The present invention relates to a polypeptide comprising an amino acid having a bicyclo [6.1.0] non-4-yn-9-ylmethanol (BCN) group, and in particular, the BCN group exists as a residue of a lysine amino acid. Relates to polypeptides. The invention also relates to a method for producing a polypeptide comprising a BCN group, said method comprising genetically incorporating an amino acid comprising a BCN group into the polypeptide. The present invention also provides amino acids comprising bicyclo [6.1.0] non-4-yn-9-ylmethanol (BCN), in particular bicyclo [6.1.0] non-4-in-9-ylmethanol ( It also relates to an amino acid that is BCN) lysine. In addition, the present invention relates to PylRS tRNA synthetase comprising mutations Y271M, L274G, and C313A.