monitoring of clone type of mantle cell lymphoma in the peripheral blood after immunization transplant

摘要

The present invention, by the post-treatment analysis of clone type profile from a sample of the patient's blood, immune transplant patients and it relates to a method of monitoring the mantle cell lymphoma residual disease in. In some embodiments, the method of the present invention, by immunizing transplant the patient by autologous T cells (a) vaccines stimulation process for treating a patient; (b) to the patient, B cells and / or a step of obtaining a peripheral blood sample containing cell free nucleic acid; and a step (c) for amplifying the molecules of the cell free nucleic acid nucleic acid and / or said sample of B cells from said sample, said nucleic acid molecule, To form a (d) Clone-shaped profile, the step of sequencing the amplified said nucleic acid molecule; a process comprising recombinant DNA sequences derived from immunoglobulin genes and from (e) the clones shaped profile, a line including biological clone type and includes one or more patient-specific clones type determining the presence and / or level of that associated with the mantle cell lymphoma.BACKGROUND