METHODS FOR INTRODUCING NICK INTO DOUBLE-STRANDED DEOXYRIBONUCLEIC ACID AND PROTEINS HAVING NICKING ENZYME ACTIVITY

摘要

PROBLEM TO BE SOLVED: To provide a method for introducing a nick at a specified site in a base sequence of double-stranded deoxyribonucleic acid.;SOLUTION: The method for introducing a nick at a specified site of the base sequence of a double-stranded deoxyribonucleic acid (dsDNA) of the invention introduces a nick at a thymine/guanine (T/G) mismatch site in the dsDNA, and/or at a site in the specified base sequence in the dsDNA.;COPYRIGHT: (C)2015,JPO&INPIT