How carbon dioxide and to produce Actinobacillus pleuropneumoniae toxins ApxI or ApcIII in liquid culture medium under supply of air enriched

摘要

The current invention pertains to a method to produce RTX-toxins ApxI or ApxIII by culturing Actinobacillus pleuropneumoniae bacteria in a liquid culturing medium that supports growth of the bacteria, characterized in that air is passed through the medium, wherein the air has a carbon dioxide content above normal atmospheric level to increase RTX-toxin production during the production phase of the RTX-toxins.