random double-strand breaks in DNA that use the enzyme

摘要

Non unique nuclease and enzyme manufacturing ones which include with the T7 ended I mutation body at unit ratio under the 1:200 are stated. Any this enzyme manufacturing things can be used in order to produce two these chain DNA fragments of the size which is suited for DNA arrangement decision. End of the said fragment the adapter or can be decorated in order to connect the individual nucleotide easily in the chain of one side of said two these chain DNA fragments according to need.