Immunoassay using surface plasmon and fluorescence resonance energy transfer .

摘要

Providing immunoassay using SPFS and [Problems] FRET. [MEANS FOR SOLVING PROBLEMS] The present invention provides an immunoassay method for determining the amount of antigen in a sample using a second antibody conjugated to a fluorescent dye serves as an acceptor molecule and the primary antibody conjugated to a fluorescent dye as a donor molecule, At least, the step (a): causing an antigen-antibody reaction between the two antibodies primary and secondary antibodies of the antigen in the analyte, a metal immunocomplexes antigen is sandwiched by both antibodies step of fixing the thin film substrate, the step (b): for the immune complexes formed by irradiating a laser beam surface plasmon excitation fluorescence by (SPFS), donor molecules excited fluorescence of the primary antibody I a step of measuring the fluorescence intensity of the acceptor molecules emitted by fluorescence resonance energy transfer (FRET) from the dye. Assay kit and system of the present immunoassay for also included.