method for measuring the concentration of autoantibodies using an enzyme immunoassay

摘要

The natural self antibody which the biotin conversion antigen treats the solid phase for the aforementioned physical sorption with the biotin conversion protein in order block the non unique binding site of the solid phase for the physical sorption making use of the solid phase for the physical sorption which sutoreputoabijinkoteingu is done, in the human biological fluid it regards quantitative measuring method. The conjugate content solution reacts with 1 genera or everything of human immunoglobulin ISOTYPE monoclonal or poly clonal includes the enzyme indicator antibody. It dilutes the subject biological fluid beforehand with the buffer which includes with the protein block the non unique join of the solid phase for the physical sorption and the substrate which prevents the resolution of the natural self antibody which is in the midst of heating treating it heats treats. The solid phase for the contrast physical sorption is used vis-a-vis each biological fluid subject sample. Using the monoclonal antibody and the polyclonal antibody for the antigen, it calculates the number of natural self antibodies, making use of the analytical curve which it plots. Choice figure Drawing 1