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Industrial purification of biologically active algal toxins
Industrial purification of biologically active algal toxins
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机译:生物活性藻毒素的工业纯化
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摘要
This invention is related to a method for the industrial purification of biologically active phycotoxins that comprises providing a suitable amount of a phycotoxin source; if the phycotoxin source is a pellet, said pellet is lysed using suitable methods; the material from the cyanobacterial lysis is subjected to a cold extraction; obtaining a concentrate of the aqueous phase obtained in the previous step or the culture medium of the first step; centrifugating the concentrate to obtain a supernatant; passing the supernatant through a diatomaceous earth column and obtaining a phycotoxin-containing eluate; passing the eluate obtained in the previous step through activated charcoal columns; the eluate from the previous step is passed again through a diatomaceous earth column; the eluate from the previous step is left in an aqueous phase; the partially purified extract from the previous step is subjected to a preparative HPLC in several steps to obtain the pure biologically active phycotoxin.
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