macrolide antibiotic resistance detection method and kit for detecting mutations bacteria

摘要

To provide quickly and appropriately macrolide antibiotic resistance detection method of the mutant bacteria . The macrolide antibiotic-resistant mutant bacteria, detected by real-time PCR in the first hydrolysis probe and the second hydrolysis probe. The first hydrolysis probe is part of the sequence is composed by the LNA, a second hydrolysis probes, the 5 'end is labeled with a reporter fluorescent dye, 3' terminus quencher non-fluorescent dye and MGB (Minor Groove Binder) with is labeled. first, the fluorescence derived from the second both hydrolysis probes is observed when the macrolide antibiotic sensitive bacterium is detected. When the macrolide antibiotic-resistant mutant bacterium is detected, only the fluorescence from the second hydrolysis probes is observed.