Method for producing stable isotope-labeled target peptide fragments in mass spectrometry

摘要

It is an object to provide a method for producing a stable isotope-labeled target peptide fragment in mass spectrometry, which can be produced inexpensively and easily. As a solution, a stable isotope labeling of a ligation DNA comprising a tandem-binding DNA in which two or more DNAs encoding one or more target peptide fragments are bound to a tandem and a DNA encoding a peptide fragment for concentration measurement. A step of preparing a stable isotope-labeled protein by expressing it in a system in which an amino acid is present; a stable isotope-labeled protein is fragmented with trypsin, and a stable isotope-labeled concentration measurement peptide fragment and a stable isotope-labeled target Step of preparing peptide fragment; Step of quantifying peptide fragment for measuring stable isotope labeling concentration with liquid chromatograph-tandem mass spectrometer (LC / MS / MS); Quantifying value of peptide fragment for measuring stable isotope labeling concentration From the step of calculating the concentration of the stable isotope-labeled target peptide fragment Producing stable isotope-labeled target peptide fragments in analysis.