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METHOD OF IN VITRO DIFFERENTIATION OF MOTOR NEURON PROGENITORS (MNPS) FROM HUMAN INDUCED PLURIPOTENT STEM CELLS AND CRYOPRESERVATION OF MNPS
METHOD OF IN VITRO DIFFERENTIATION OF MOTOR NEURON PROGENITORS (MNPS) FROM HUMAN INDUCED PLURIPOTENT STEM CELLS AND CRYOPRESERVATION OF MNPS
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机译:从人诱导的多能干细胞中体外分离运动神经元祖细胞(MNPS)和冷冻保存MNPS的方法
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摘要
Methods are disclosed for the initiation and differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) into motor neuron progenitor cells (MNPs). Methods are also disclosed for the cryopreservation of MNPs. The methods particularly relate to the simple, efficient, scalable, and reproducible generation, and subsequent frozen maintenance, of MNPs for downstream therapeutic applications. The methods can be used for the production of MNPs from various lines of hESCs and iPSCs.
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