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PLASMID VECTOR, METHOD FOR DETECTING GENE PROMOTER ACTIVITY, AND ASSAY KIT

机译:质粒矢量,基因启动子活性的检测方法和检测试剂盒

摘要

According to one embodiment, a first gene encodes a reporter protein. The first gene is disposed at the downstream of the gene promoter. A second gene is disposed at the downstream of the gene promoter and encodes a replication origin-binding protein. An internal ribosome entry site is disposed between the first gene and the second gene. The transcription termination signal sequence encodes a signal for terminating the transcription of the first gene and the second gene. A replication origin sequence is recognized by the replication origin-binding protein.
机译:根据一个实施方案,第一基因编码报道蛋白。第一个基因位于基因启动子的下游。第二个基因位于基因启动子的下游,并编码复制起点结合蛋白。内部核糖体进入位点位于第一基因和第二基因之间。转录终止信号序列编码用于终止第一基因和第二基因的转录的信号。复制起点序列被复制起点结合蛋白识别。

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