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Cutoff Point Delta Ct. Method for HER2 PCR Testing in Breast Cancer
Cutoff Point Delta Ct. Method for HER2 PCR Testing in Breast Cancer
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机译:截止点增量Ct。乳腺癌中HER2 PCR检测的方法
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摘要
The present invention is related to an improved method for HER2 gene test by using quantitative real-time PCR (Polymerase Chain Reaction) technique. Our invention streamlines test process, and incorporates quality control for each major step, including sample, reagent, operation, and data report. We eliminate the need for reference genes which is hard to standardize in HER2 PCR test. We develop a cutoff reference point by using the statistical mean of tumor tissue population, and adopt a simplified scoring scheme for evaluation of HER2 status. Our invention produces consistent result across machines and labs, and has proven to be clinically successful in HER2 test.
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