METHOD USING FLUORESCENT TURN-ON PROBES FOR CELL-SPECIFIC DETECTION

摘要

The invention provides method for imaging the activity of enzymes or bioactive molecules in cells using fluorescent probes with aggregation-induced emission properties and excited-state intramolecular proton transfer properties. The fluorescent probes prepared according to the invention are those of formula (I),; ;wherein W, X, Y, Z have the meaning as described in the description, R is a reactive group that can interact with enzymes and other bioactive molecules, Linker is a single bond or a combination of chemical bonds linking the targeting group T to the probe molecule, and T represents a targeting group that has an ability to interact with an organelle.