METHODS FOR IDENTIFICATION OF ORGANISMS, ASSIGNING READS TO ORGANISMS, AND IDENTIFICATION OF GENES IN METAGENOMIC SEQUENCES

摘要

The present application provides a computational procedure that receives metagenomic or metatranscriptomic sequence reads. Briefly, the computational procedure comprises masking or removal of low-complexity, highly conserved and vector sequences; read mapping using homology searches to a database comprising genomes; post-processing the search results to identify organisms that are present in the data and remove false positives. Functional annotation are propagated from the mapped genomes. The output comprises inferred mapping of input reads to organisms, genes and functions of the reference database.