SYSTEMS AND METHODS FOR IMAGING AT HIGH SPATIAL AND/OR TEMPORAL PRECISION

摘要

Various aspects of the present invention are generally directed to systems and methods for imaging at high spatial and/or temporal resolutions. In one aspect, the present invention is generally directed to an optical microscopy system and related methods adapted for high spatial and temporal resolution of dynamic processes. The system may be used in conjunction with fluorescence imaging wherein the fluorescence may be mediated by voltage-indicating proteins. In some cases, time resolutions may be enhanced by fitting predefined temporal waveforms to signal values received from an image. The system may also contain a high numerical aperture objective lens and a zoom lens located in an imaging optical path to an object region. Other aspects of the present invention are generally directed to techniques of making or using such systems, kits involving such systems, manufactured storage devices able to implement such systems or methods, and the like.