Mass spectrometry imaging method for detecting and quantifying a target molecule in a tissue sample

摘要

The invention relates to a method for identifying and quantifying by mass spectrometry imaging at least one target analyte in a tissue sample, comprising the following steps:a) depositing the tissue sample to be analyzed on a support;b) analyzing the sample by mass spectrometry imaging, so as to obtain the mass spectrum of the target analyte in said tissue sample;c) weighting a signal associated with the mass spectrum of the target analyte in said tissue sample by a tissue extinction coefficient (TEC) specific to the target analyte and to the tissue sample which is obtained by using a control tissue sample; andd) using the weighted signal of the target analyte to determine the quantity of target analyte in the tissue sample.