Method and compositions for the genetic control of Insect pests in cotton Plants Through Gene Silencing of Chitin Synthases

摘要

This application is related to the control of Pest infestation through inhibition or reduction of gene expression of Chitin synthase. The application also provides methods and compositions for the control of pests, through feeding of one or more Molecules of double stranded rna provided by this.The application also describes a method of obtaining transgenic plants expressing Double stranded rna molecules. This application is mainly used for Cotton plants.Claim 1: isolated Nucleic Acid Molecule, which is selected from the group consisting of: (a) an isolated Nucleic Acid Molecule comprising a Nucleic Acid Sequence substantially similar to the selected sequences of SEQ ID no. 1, SEQ ID n SEQ ID no. 2, no. 3, SEQ ID no. 4SEQ ID SEQ ID no. 5 and no. 6; (b) an isolated Nucleic Acid Molecule that hybridizes with a Nucleic Acid Sequence indicated by the selected sequences of SEQ ID no. 1, SEQ ID no. 2, SEQ ID SEQ ID no. 3, no. 4, SEQ ID SEQ ID no. 5 and no. 6, under Cleaning conditions 5X SSCThe 50% formamide and 42; during 10 min; (c) Fragment of at least 19 contiguous Nucleotide sequence of a Nucleic Acid selected from the group of SEQ ID no. 1, SEQ ID no. 2, SEQ ID SEQ ID no. 3, no. 4, SEQ ID SEQ ID no. 5 and no. 6, where ingestion for Plant Pest Beetles S, a ribonucleotide sequence of double Strand,It includes at least a complementary Strand of the fragment mentioned, Inhibits or reduces the Proliferation of the Plague mentioned; and (d) complement of the sequence of (a), (b) or (c).