Provided is a modified Family 1 carbohydrate binding module (CBM) comprising amino acid substitutions at one or more of positions 10, 11, 12, 14, 17, 21, 24, 29, 31, 33, and 37, said position determined from alignment of a Family 1 CBM amino acid sequence with SEQ ID NO: 30, and exhibiting from about 50% to about 99.9% amino acid sequence identity to SEQ ID NO: 30. Also provided are modified glycosidase enzymes comprising the modified Family 1 CBM, genetic constructs and genetically modified microbes for expressing the modified Family 1 CBM or modified glycosidase enzyme. The modified Family 1 CBM confers reduced lignin binding and/or increased hydrolyzing activity in the presence of lignin to the modified glycosidase enzyme, which may be used in a process for hydrolyzing cellulose or hemicellulose in the presence of lignin.
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机译:提供了修饰的家族1碳水化合物结合模块(CBM),其在位置10、11、12、14、17、21、24、29、31、33和37中的一个或多个上包含氨基酸取代,所述位置由比对确定具有SEQ ID NO:30的家族1 CBM氨基酸序列的序列,并且与SEQ ID NO:30表现出约50%至约99.9%的氨基酸序列同一性。还提供了包含修饰的家族1 CBM的遗传修饰的糖苷酶表达修饰的Family 1 CBM或修饰的糖苷酶的重组体和经遗传修饰的微生物。在木质素存在下,修饰的家族1 CBM赋予木质素与修饰的糖苷酶的减少的木质素结合和/或增加的水解活性,其可用于在木质素存在下水解纤维素或半纤维素的方法中。
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