Method and kit for simultaneous separation of mononuclear cells, and leucu00d3citos granulu00d3citos total human blood or animals

摘要

Method and kit for simultaneous separation of mononuclear cells, and total blood granulu00d3citos leucu00d3citos human or animals. The present invention refers to a method that leads to a kit lens purification simultaneously or not of mononuclear cells and granulocytes. This method allows the separation from crude blood, bone marrow or cord blood of neonates.The invention is constituted by mixing proportions of gradients defmidas + B and C + D (Figures 1 and 2), in different densities and concentrations varied, which allows the purification cell after time and centrifugation previantente defined and result in one, two or more rings interface u00e1sicos composed of leukocytes with purity higher than 95%.The present invention is to replace the import of gradients and presents a low cost