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Process for obtaining ethanol by bioprocessing glycerol derived from biodiesel using wild escherichia coli and atcc 25922

机译:通过使用野生大肠杆菌和atcc 25922对生物柴油中的甘油进行生物处理获得乙醇的方法

摘要

Process for obtaining ethanol by bioprocessing biodiesel-derived glycerol using wild escherichia coli and atcc 25922. The present invention relates to the production of ethanol from anaerobic fermentation of crude glycerol by strains of the wild (sp) escherichia coli bacterium. Broken stream in Guarapuava - Parana, Brazil (geographic coordinates of latitude 25 ° 21 '06 "to 25" 24´22 "s and 51 ° 25' 03" to 51 ° 31'02 "w), and" certified "(atcc 25922 Ethanol is obtained by this process by fermentation using different nutrients and using certain strains of E. coli certified and sp using crude glycerol in ph 12, specific mass of 1.029 g / cm³ and percentage of ethanol and methanol (less than The pH of the nutrient media was adjusted to 7. Biotransformation by both Escherichia coli sp and Escherichia coli atcc 25922 allows the minimization of the environmental problem generated by the glycerol co-product. l from biodiesel plants. The ethanol produced can be used industrially either in the transesterification process itself for biodiesel production or as bioethanol for cleaning, sterilization or as automotive biofuel.
机译:通过使用野生大肠杆菌和atcc 25922对生物柴油衍生的甘油进行生物处理来获得乙醇的方法。本发明涉及通过野生(sp)大肠杆菌细菌菌株的粗制甘油的厌氧发酵生产乙醇。瓜拉普阿瓦(Guarapuava)-巴西帕拉纳(Parana)的断流(纬度25°21 '06“至25” 24´22“ s和51°25'03”至51°31'02“ w”的地理坐标),并且经过“认证”(atcc 25922乙醇是通过以下过程获得的:通过使用不同的营养物质发酵,并使用经认证的某些大肠杆菌菌株,并使用pH值为12的粗甘油和1.029 g /cm³的比重以及乙醇和甲醇的百分比(小于pH将营养培养基调整为7。大肠杆菌sp。和大肠杆菌atcc 25922的生物转化可将甘油副产物从生物柴油工厂中产生的环境问题减至最小,所产生的乙醇可在酯交换过程中工业使用本身可用于生产生物柴油或用作清洁,灭菌或用作汽车生物燃料的生物乙醇。

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