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Variant yeast, and methods for isolating a yeast, and for creating a mutant yeast strain.
Variant yeast, and methods for isolating a yeast, and for creating a mutant yeast strain.
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机译:变体酵母,以及分离酵母和产生突变酵母菌株的方法。
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摘要
Disclosed herein are materials and methods for creating and/or isolating variants of yeasts especially variants of Saccharomyces cerevisiae that can grow on sugars other than D-glucose in the presence of amounts of 2-deoxy-glucose and/or D-glucose that inhibit most strains of yeast from growing on sugars other than D-glucose. Selection media that can be used to isolate such variants include pentose sugars such as D-xylose, L-glutamine and 2-deoxy-glucose. Mutations in the Grr1 and Red genes in some strains also produce variants that can grow on sugars including the pentose D-xylose in the presence of 2-deoxy-glucose.
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