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Use of Salmonella enterica ssp. enterica serovar Typhimurium antigens for serological differentiation of infected and vaccinated pigs

机译:使用沙门氏菌小肠沙门氏菌。肠炎血清鼠伤寒抗原用于感染猪和疫苗接种猪的血清学区分

摘要

ufeffu0159eu0161enu00ed describes a method of modulation of pigs infected with salmonella enterica ssp. enterica serovar typhimurium from swine vaccinated with the vaccine based on inactivated culture of salmonella enterica ssp.enterica serovar typhimurium in which the serum sample elisa provides a method or westernblot antibody levels against at least one selected from the group nativnu00edmu or rekombinantnu00edmu protein sseb, gene id: 1252916; sipb, gene id: 1254408 and sipd, gene id, and at least one 1254406 nativnu00edmu or rekombinantnu00edmu protein selected from the group of flic gene id: 1253480 and yfgl, gene id: 1254042.infected animals are identified so that they have higher levels of antibodies against the protein from vaccinated animals sseb or sipb or sipd and, at the same time, have lower levels of antibodies against the protein flic or yfgl, by contrast, animals vaccinated with the identified,they compared with animals infected with higher antibody levels against protein flic or yfgl and, at the same time, have lower levels of antibodies against the protein sseb or sipb or sipd. serological differentiation of vaccinated animals infected animals is necessary for the implementation of the recovery, the aim of which is to reduce the percentage of salmonella positive slaughter pigs.another reason for the need of modulation from the animals vaccinated animals infected is to exclude false positive vaccinated pigs in the estimation promou0159enosti breeds pigs salmonella ssp. using the already established serological methods in some countries of western europe.
机译:描述了一种调节感染了肠炎沙门氏菌的猪的方法。基于灭活的沙门氏菌肠炎沙门氏菌的疫苗接种疫苗的猪的肠炎血清型鼠伤寒沙门氏菌,其中血清样品elisa提供了针对至少一种选自天然蛋白或重组蛋白的方法或westernblot抗体水平sseb,基因ID:1252916; sipb,基因ID:1254408和sipd,基因ID,以及至少一种1254406 nativn u00edmu或rekombinantn u00edmu蛋白选自flic基因ID:1253480和yfgl,基因ID:1254042。免疫接种的动物sseb或sipb或sipd的蛋白质的抗体水平较高,同时flic或yfgl的蛋白质的抗体水平较低,相比之下,已鉴定疫苗的动物与被感染的动物相比较高的针对蛋白质flic或yfgl的抗体水平,同时针对较低的蛋白质sseb或sipb或sipd的抗体水平。接种动物的血清学差异是实现恢复所必需的,其目的是减少沙门氏菌阳性屠宰猪的百分比。需要对感染动物进行调节的另一个原因是要排除假阳性疫苗猪在估计促销中繁殖出猪沙门氏菌属。使用西欧一些国家已经建立的血清学方法。

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