CYTOSKELETAL GENE BASED SPECIFIC PRIMERS FOR COLLETOTRICHUM FALCATUM DETECTION

摘要

The present invention relates to the use of novel cytoskeletal gene- actin based primers CF-ACT1 and CF-ACT2 in the detection of Colletotrichum falcatum. This method is useful in detecting the pathogen infection in symptom less tissue of any plant, detecting pathogen in soil, confirming the presence of pathogen in the mixed state of plant disease/culture, monitoring progress of pathogen in plant and screening plants for disease resistance and identification of newer races of C. falcatum, its origin and virulence group. This method is unique in detecting C. falcatum and offers advantages like accuracy, rapidity, reliability and applicability for high throughput analysis.