Nanomaterial based novel growth medium for enhanced efficiency of agriculturally beneficial bio-agents and plant - growth promoting rhizobacteria

摘要

This invention describes the modification of growth medium for enhanced efficiency of agriculturally beneficial bio-agents and plant growth promoting rhizobacteria. The present study was undertaken to investigate the Bacillus thuringiensis growth on modified medium. The modified medium was prepared with the addition of Nano materials of Zn and Cu to Luria Bertani broth which is specific for Bacillus thuringiensis. Three different concentrations were used to analyse the variation in culture of Bacillus thuringiensis. Bacillus thuringiensis cell count was recorded after 24hours. The culture with Nano materials of Zn and Cu was inoculated on solid media which is known as Luria Bertani agar to observe the number of colonies formed. The results revealed that there was substantial increase in cell count as well as Colony Forming Units (CFU) when nano materials of Zn and Cu is added at lower concentrations (50ppm). Whereas the growth of Bacillus thuringiensis suppressed at high concentrations(50ppm &100ppm) of Nano ZnO and CuO.