The present application discloses a(n) (isolated) nucleic acid sequence comprising a nucleotide sequence selected from (a) SEQ ID NO: 1 or a fragment thereof wherein said fragment comprises at least 400 consecutive nucleotides of SEQ ID NO: 1 and has seed specific promoter activity; (b) a nucleotide sequence with at least 80% sequence identity with SEQ ID NO: 1 and having seed specific promoter activity; (c) a nucleotide sequence hybridizing under stringent conditions to the nucleotide sequence of (a) or (b); and (d) a nucleotide sequence complementary to the nucleotide sequence of any one of (a) to (c). Further disclosed herein is a chimeric gene comprising the (isolated) nucleic acid described herein operably linked to a nucleic acid coding for an expression product of interest and optionally a transcription termination and polyadenylation sequence. Also disclosed herein are a vector a transgenic plant cell a transgenic plant and a seed as characterized in the claims. Methods disclosed herein relate to the production of a transgenic plant growing cotton producing a seed effecting seed specific expression of a product in cotton and of altering fiber properties in a cotton plant as characterized in the claims.
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机译:本申请公开了包含选自(a)SEQ ID NO:1或其片段的核苷酸序列的(n)(分离的)核酸序列,其中所述片段包含SEQ ID NO:1的至少400个连续核苷酸。比启动子活性(b)与SEQ ID NO:1具有至少80%序列同一性并且具有种子特异性启动子活性的核苷酸序列; (c)在严格条件下与(a)或(b)的核苷酸序列杂交的核苷酸序列; (d)与(a)至(c)中任一项的核苷酸序列互补的核苷酸序列。本文进一步公开了一种嵌合基因,其包含与编码目的表达产物以及任选地转录终止和聚腺苷酸化序列的核酸可操作地连接的本文所述的(分离的)核酸。本文还公开了如权利要求中所述的载体,转基因植物细胞,转基因植物和种子。本文公开的方法涉及转基因植物生长的棉花的生产,所述棉花产生影响种子在棉花中的产品的特异性表达并改变棉花植物中的纤维特性的种子,如权利要求中所述。
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