Novel luminescent lanthanide chelates with enhanced excitation properties

摘要

The present application discloses a luminescent lanthanide chelate comprising one or more chromophoric moieties of the formula (I) or of the formula (III) wherein R R and R each independently are selected from carbon containing substituents forming a C O bond with the neighbouring oxygen atom R and R each represent a bond between the chromophoric moiety and other moieties of the chelate and Ln is a lanthanide ion as well as the corresponding luminescence lanthanide chelating ligand. The application also discloses a detectable molecule comprising a biospecific binding reactant (such as an antibody) conjugated to the luminescent lanthanide chelate as well as a method of carrying out a biospecific binding assay the use of such a detectable molecule in a specific bioaffinity based binding assay utilizing time resolved fluorometric determination of a specific luminescence and a solid support material conjugated with the luminescent lanthanide chelate.