A process for rapidly screening enzyme and enzyme mixtures for the hydrolysis of ligno cellulosic biomass

摘要

The invention is to a novel method for evaluating the hydrolysis conversion of a enzyme or enzyme mixture relative to a specific ligno cellulosic biomass. The method evaluates the hydrolysis conversion of a enzyme or enzyme mixture relative to a specific ligno cellulosic biomass by preparing a pretreated ligno cellulosic biomass suspension a buffer solution and an enzyme or enzyme mixture solution; dispensing a portion of one or more of the three components into a plurality of test vials subjecting one or more of the plurality of test vials to varying incubation periods reducing the rate of incubation at the end of each incubation period and analyzing the resulting samples for dissolved sugars to determine the rate of reaction.