B and t cell specific peptides of leptospiral protein lk90 for the diagnosis leptospirosis

摘要

The present invention pertains to three peptides “HSSNNSVATC”, “SNAQKNQGNC” and “DHHTQSSYTC” with greater BCP red and VaxiJen score were selected for chemical synthesis. Further these epitopes were found to be highly conserved among all pathogenic Leptospira species by multiple sequence alignment. The three peptides were synthesized with biotin at the N-terminal linked to the peptide sequence through a spacer sequence of “SGSG” to improve the solubility of the peptide and the ease to perform ELISA.