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A method for fabricating bio-artificial skin graft by loading cells on cholecyst extracellular matrix for wound healing applications
A method for fabricating bio-artificial skin graft by loading cells on cholecyst extracellular matrix for wound healing applications
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机译:通过将细胞加载到胆囊细胞外基质上以用于伤口愈合的方法来制造生物人工皮肤移植物的方法
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摘要
pThis invention relates to a process for the preparation of an artificial skin-graft comprising the steps of providing HaCaT cells (immortal keratinocyte cell line), adding trypsin thereto followed by incubation of the cells to detach the cells, followed by neutralisation of the trypsin, isolating scaffolds from cholecyst derived extracellular matrix and subjecting the same to sterilization followed by seeding the HaCaT cells on the scaffolds and maintaining the HaCaT culture on the scaffolds to obtain the skin-graft./p
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