BisN'-(2-hydroxy-kO-benzylidene)pyridine-4-carbohydrazidate(-1)-k2N',Oiron(III) nitrate - water (2/3) and process for cultivation of microalga Porphyridium cruentum with its use

摘要

The invention relates to chemistry and biotechnology, in particular to the synthesis of a new coordinative compound of iron(III) with antioxidant properties that can be used in the food industry and in medicine, and to a process for cultivation of microalga Porphyridium cruentum with its use.According to the invention, a coordinative compound - bis¢N'-(2-hydroxy-kO-benzylidene)pyridine-4-carbohydrazidate(-1)-k2N',O]iron(III) nitrate - water (2/3) is claimed.A process for cultivation of microalga Porphyridium cruentum is also claimed, which consists in that microalga is cultivated on a nutrient medium containing, g/L: NaNO3 - 5.0; NaCl - 7.0; KCl -7.5; MgSO4·7H2O - 1.8; Ca(NO3)2·4H2O - 0.15; KBr - 0.05; KI - 0.05; K2HPO4 - 0.2; ZnSO4·5H2O - 0.00002; CuSO4·5H2O - 0.00005; MnSO4·5H2O - 0.0003; H3BO3 - 0.0006; MoO3 -0.00002; NaVO3 - 0.00005, the compound bis¢N'-(2-hydroxy-kO-benzylidene)pyridine-4-carbohydrazidate(-1)-k2N',O]iron(III) nitrate - water (2/3) - 0.01…0.012 g/L and distilled water up to 1 L, having the pH 6.8…7.2; at a temperature of 23…25°C, an illumination of 2000…3000 lx/cm2, with periodic stirring.The result consists in increasing the content of phenols in the ethyl extract obtained from microalga biomass.