ESCHERICHIA COLI STRAINS WITH A HIGH CAPACITY OF PRODUCING PLASMIDS AND THEIR USE IN THE PRODUCTION OF DNA VACCINES.

摘要

The present invention refers to novel Escherichia coli W3110 strains known as WGMC, WGM and WGME that have the mutations AptsG AmanX AmgIABC, AptsG AmanX and AptsG AmanX AnagE, respectively. As a result of these mutations, the strains show a reduction in the specific speed of glucose consumption. This modification causes a reduction in the specific speed of growth and the specific speed of acetate production. The aforementioned strains and further control strains were transformed with the pHN plasmid, which is used as a DNA vaccine. The culturing results, at a flask level, indicate that the WGMC, WGM and WGME strains shown a value of YP/X 3.2, 2.4 and 2.1 times higher than that observed in the strain W3110 transformed with the same plasmid.