Diagnostic test based on amino-modified nano-gold phosphorylated nucleotides conjugated to identify drug-resistant bacterial strains and the method for its preparation

摘要

a diagnostic test based on amino modified nanozlota sprzezonego with fosforylowanymi nukleotydami to identify lekoopornosci bacterial strains.there are nitrocelulozowej membrane type lateral flow nasaczonej way ahead on points solution protein wiazacego biotyne in amounts of from 0.005 mg / mm2 to 0.1 mg / mm2 suspended in 10% aqueous solution of ethanol and reagent request detector 25. g nanoczastek au modified ammonium sprzeganych kowalencyjnie with probe hybrydyzacyjna liaison officerthrough the free group in 1 - 10 of phosphatic nm with 1 - 10 pmolami probe hybrydyzacyjnej of biotin.process for the production of a diagnostic test based on amino modified nanozlota sprzezonego with fosforylowanymi nukleotydami to identify lekoopornosci bacterial strains is,in the first stage membrane nitrocelulozowa type lateral flow nasacza's way ahead on points wiazacego biotyne in protein solution of from 0005 mg / mm2 to 0.1 mg / mm2 suspended in 10% aqueous solution of ethanol.in the second stage is inkubacje modified liaison aminowym nanoczastek au of stezeniu 25? g / ml with the probe hybrydyzacyjna with phosphatic free group containing from 1 to 10 nm oligonukleotydu, after the sprzegania powstale nanoczastki au being concentration in process of spinning 14 400 x g for 30 minutes.the supernatant and the precipitate suspended rejects it at 100? l - 200? l deionised distilled water, then the solution is subjected to concentration again,the nanoczastek au suspended in 39 - 109. deionised distilled water and added to l l ul solution biotynylowanej probe hybrydyzacyjnej zawierajacej 1 - 10 pmoli of the oligonucleotide.then in the third stage to 10 - 30? l the detector reagent adds to 10? l shaggy bacterial culture, mieszanine reactionary placed in bath water temperature in the 90 - 100u00b0c during 3 - 5 minthen leaves to ostygniecia to room temperature and 10 - 20? l mixture moving in with immobilizowanym reaction membrane bialkiem wiazacym biotyne.