Three-dimensional scaffold for cultured neuronal and method for producing the scaffold

摘要

scaffold for three-dimensional cell culture obtained by the differentiation of neuronal stem cells, in the form of a porous hydrogel, with synthetic polymer is characterized by the fact that contains poly (2-hydroxyethyl methacrylate), poly (diakrylan poly (ethylene glycol)) and p oli (chloride metakryloetylotrimetyloamoniowy)with the scaffolding is to 70% in relative to the dry matter of the polymers.the invention discloses also a way to manufacture three-dimensional scaffold cell culture obtained by the differentiation of neuronal stem cells, is that a 2 - hydroxyethyl methacrylate mixture of poly (ethyl glycol diakrylanem) as a measure. ciuju0105cym, containing more than metakryloetylotrimetyloamoniowy chloride,preferably in the form of an aqueous solution and porogen based mixture, after being placed in the container, is subjected to polymerization and sieciowaniu using ionizing radiation in the form of a stream of electrons from the accelerator or cobalt isotope 60co gamma radiation with until zaab sorbowania this dose to the appeared gelthen the produced material is removed from the packaging, washed in deionised water and subjected to sterilisation.