Fluorometric immunoassay for the determination of antibodies against double-stranded DNA

摘要

the invention belongs to the field of immunological methods, more specifically in the field of methods for the determination of anti dsdna, for the purposes of diagnosis of chronic autoimmune diseases, such as. systemic lupus erythematosus (sle).with fluorometriu010dno immune method of anti - dsdna is solved the technical problem of design methods of these autoantibodies, which will be faster, cheaper and less toxic than the standard method farr - ria using the same diagnostic specificity (100%) and improved diagnostic obu010dut capacity (about 3 percent).determination of anti dsdna, based on fluorescence detection in two fractions of samples, it is in the supernatant and in the pattern of precipitation, in which are located the immune complexes consisting of added dsdna, and anti - dsdna present in the serum of the patient, and the fluorescence is detected due to the binding of fluoresce ntnih dyes with dsdna, so coupled in the immune complex, as well as free dsdna.the method of the invention allows to obtain reliable results, fast sample analysis and the results leu010deu010demu doctor, the use of human and the environment of hazardous chemicals and reduce testing costs, compared with older radioimunsko farr - ria method.