Transgenic plants expressing SPIC or modified intein proteins and related method

摘要

A plant, or part of the plant, seedling, tissue, cell, subcellular fraction, seed, seedling, protoplast, offspring or recombinant offspring, characterized in that it has an expression construct that encodes a modified protein comprising a target protein and an intein sequence of Pyrococcus spp. polymerase. (pol Psp) variant that has a methionine in amino acid residue 534, in which the intein sequence pol Psp variant is fused within the target protein in a position such that the presence of intein substantially reduces the activity of the protein modified, and where the target protein is a degrading lignocellulosic protein and the variant pol Psp intein sequence is thermally inducible to produce cis-splicing of the plant-modified protein, plant part, seedling, tissue, cell, subcellular fraction, seed, seedling, protoplast, offspring or recombinant offspring that have a temperature of 50 ° C or higher.